SINGLE-CELL TRANSCRIPTOME REVEALS STAPHYLOCOCCUS AUREUS MODULATING FIBROBLAST DIFFERENTIATION IN THE BONE-IMPLANT INTERFACE

Single-cell transcriptome reveals Staphylococcus aureus modulating fibroblast differentiation in the bone-implant interface

Abstract Background This study aimed to delineate the cell heterogeneity in the bone-implant interface and investigate the fibroblast responses to implant-associated S.aureus infection.Methods Single-cell RNA sequencing of human periprosthetic tissues from patients with periprosthetic joint infection (PJI, n = 3) and patients with aseptic loosening

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High-level expression of Bacillus naganoensis pullulanase from recombinant Escherichia coli with auto-induction: effect of lac operator.

Pullulanase plays an important role in specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in starch-processing industry.So far, however, the production level of pullulanase is still somewhat low from wide-type strains and even heterologous expression systems.Here the gene encoding Bacillus naganoens

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